How does Alexa Fluor 488 work?

How does Alexa Fluor 488 work?

Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 488 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

What are Alexa Fluor antibodies?

Alexa Fluor ReadyProbes Secondary Antibodies These Alexa Fluor secondary antibodies recognize IgG heavy chains and all classes of immunoglobulin light chains from either mouse or rabbit. Like other ReadyProbes reagents, these secondary antibodies are provided in a convenient dropper bottle format.

Is Alexa 488 a GFP?

1. Product The GFP-Booster Alexa Fluor® 488 is an anti-GFP Nanobody coupled to Alexa Fluor® 488. localization and dynamics in cells. However, photo-stability and quantum efficiency of GFP are often not sufficient for e.g. super-resolution microscopy (such as 3D-SIM or dSTORM) and for fixed cell samples.

Is Alexa Fluor 488 a fluorophore?

Alexa Fluor® 488 is a green fluorophore commonly used in applications such as immunolabeling, fluorescence microscopy, and flow cytometry.

Is 633 far-red?

A bright and photostable, far-red-fluorescent probe used 633 nm or 647 nm laser lines.

Can antibodies bind to other antibodies?

The secondary antibody binds to the primary antibody but not any target that is present in the specimen. Secondary antibodies bind to the heavy chains of primary antibodies so that they don’t interfere with the primary antibody binding to the target.

At what wavelength does GFP fluorescence?

509 nm
Green fluorescent protein (GFP) is the most commonly used fluorescent reporter. Initially isolated from the jellyfish Aqueoria victoria, GFP has since been engineered to generate the brighter and more photostable enhanced GFP (eGFP), with an excitation wavelength of 488 nm and emission wavelength of 509 nm.

Is Alexa Fluor 488 the same as FITC?

Molecular Probes™ Alexa Fluor® 488 dye—with nearly identical spectral properties and quantum yield as fluorescein (FITC)—produces brighter, more photostable conjugates that are ideal for imaging and other applications requiring increased sensitivity and environmentally insensitive fluorescence detection.

Which fluorophores are brightest?

It is concluded that cyanine 3.18 should be useful for multicolor fluorescence experiments and that it may be the brightest fluorophore available for single-color fluorescence immunocytochemistry.

What color is Alexa Fluor 680?

Alexa Fluor® 680 (AF680, Alexa 680) is a red-emitting synthetic fluorophore that can be excited by the 640 nm red laser and captured with a 695/40 nm bandpass filter.

How are antibodies produced?

Antibodies are produced by specialized white blood cells called B lymphocytes (or B cells). When an antigen binds to the B-cell surface, it stimulates the B cell to divide and mature into a group of identical cells called a clone.

What is the peak of Alexa Fluor 488 antibody?

Alexa Fluor® 488 – conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media, they are brighter and more photostable than FITC, Cy™2 and DyLight™ 488.

What can Alexa Fluor 488 dye conjugate to?

We offer Alexa Fluor 488 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.

What is the difference between Alexa Fluor 488 and Cy™2 and FITC?

In aqueous mounting media, they are brighter and more photostable than FITC, Cy™2 and DyLight™ 488. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye, except for protocols that include mounting in plastic mounting media ( see Cy™2 for more information ).

How do anti-fluorescent dye antibodies work?

Anti-fluorescent dye antibodies recognize specific fluorophores and, in most cases, quench their fluorescence.