What is Genipin crosslinking?

What is Genipin crosslinking?

Genipin is a water-soluble bi-functional crosslinking reagent which is extracted from gardenia fruits according to a modern microbiological process (Xu et al., 2008). It reacts promptly with chitosan and protein thus producing blue-colored fluorescent hydrogels.

What is crosslinking chitosan?

In theory, the crosslinking of chitosan is a reaction with a crosslinking agent that leads to biopolymer preservation through the formation of bonds between chitosan chains. Considering the type of a crosslinking agent applied, the crosslinking process may proceed via covalent or ionic bonds.

How does genipin work?

Genipin generates crosslinks spontaneously with protein, collagen, gelatin, chitosan, etc. For a long time, genipin has been used as a treatment for cholestasis and hepatitis in traditional Chinese medicine. It has even been used as a natural dye because of its tinting properties with proteinic textiles [10].

What is cross linking in hydrogels?

Chemical cross-linking method uses covalent bonding between polymer chains in order to produce permanent hydrogel. The cross-link formation was carried out by the addition of small cross-linkers molecules, polymer-polymer conjugation, photosensitive agents or by enzyme catalyzed reaction.

Is Genipin a fluorescent?

Genipin compound is an iridoid derivative isolated from the fruits of Gardenia jasminoides. It generates both color and fluorescence in a single reaction with biopolymers containing primary amine groups.

Is Genipin toxic?

Genipin is an excellent natural cross-linker for proteins, collagen, gelatin, and chitosan cross-linking. It has a low acute toxicity, with LD 50 i.v. 382 mg/kg in mice, therefore, much less toxic than glutaraldehyde and many other commonly used synthetic cross-linking reagents.

How do you dissolve Genipin in PBS?

Genipin is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, genipin should first be dissolved in DMSO and then diluted with the aqueous buffer of choice. Genipin has a solubility of approximately 0.25 mg/ml in a 1:3 solution of DMSO:PBS (pH 7.2) using this method.

What is cross-linking agent?

A substance that binds DNA nucleotides together and blocks DNA synthesis. In cancer treatment, DNA cross-linking agents may kill cancer cells by damaging their DNA and stopping them from dividing.

Is Genipin soluble in water?

Structure for FDB014596 (Genipin)

Property Value Source
Water Solubility 7.18 g/L ALOGPS
logP 0.07 ALOGPS
logP -0.44 ChemAxon
logS -1.5 ALOGPS

What happens when Genipin is added to chitosan?

The addition of genipin to chitosan leads to the formation of crosslinks between primary amine groups and a crosslinked network evolves [14, 15].

How do you crosslink chitosan and glucosamine?

This crosslinking proceeds in two separate reactions, first the formation of a heterocyclic compound of genipin linked to the glucosamine residue in chitosan and second a nucleophilic substitution of its ester group to form a secondary amide link with another chitosan molecule [47] [48] [49].

Is genipin a cross linking agent?

Genipin is a derivative of geniposide found in the Gardenia fruit. It is an excellent cross-linking agent for chitosan. Genipin: non-toxic cross-linking agent. Chitosan is most reactively crosslinked by covalent bonds in the presence of aldehydes such as formaldehyde, glyoxal and glutaraldehyde.

Are chitosan cross-linkers biocompatible?

… A current challenge about chitosan cross-linkers is the chemical stabilization thanks to nontoxic reagents which could lead to the modulation of the properties for a prolonged period. Thus, an alternative reagent could be used to create a stable and biocompatible cross-linked product [65].